Lysine carbamylation for enzymatic function: metal and structural requirements
نویسندگان
چکیده
منابع مشابه
Crystal structures of vertebrate dihydropyrimidinase and complexes from Tetraodon nigroviridis with lysine carbamylation: metal and structural requirements for post-translational modification and function.
Lysine carbamylation, a post-translational modification, facilitates metal coordination for specific enzymatic activities. We have determined structures of the vertebrate dihydropyrimidinase from Tetraodon nigroviridis (TnDhp) in various states: the apoenzyme as well as two forms of the holoenzyme with one and two metals at the catalytic site. The essential active-site structural requirements h...
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Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is a crucial enzyme in carbon fixation and the most abundant protein on earth. It has been studied extensively by biochemical and structural methods; however, the most essential activation step has not yet been described. Here, we describe the mechanistic details of Lys carbamylation that leads to RuBisCO activation by atmospheric CO(2)....
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The reduction of nitrite to nitric oxide has been shown to require DPN or TPN, FAD or FMN,’ copper, and iron as electron carriers. This was determined by the stimulation of nitrite disappearance as well as by increased nitric oxide formation. Evidence was also presented indicating that the reduction of NO to Nz was stimulated by the same cofactors. The evidence was based on the stimulation of N...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations and Advances
سال: 2014
ISSN: 2053-2733
DOI: 10.1107/s2053273314096983